Air-pollutants containing poisonous particulate issues (PM) deposit within the respiratory tract and will increase microbial infections. Nonetheless, the mechanism by which this happens just isn’t properly understood. This research evaluated the impact of city particles (UP) on Streptococcus pneumoniae (pneumococcus) in vitro biofilm formation, colonization of human center ear epithelium cells (HMEECs) in addition to mouse nasal cavity and its transition to the center ear and lungs.
The in vitro biofilms and planktonic progress of S. pneumoniae had been evaluated in metallic ion free medium within the presence of UP. Biofilms had been quantified by crystal violet (CV) microplate assay, colony forming unit (cfu) counts and resazurin staining. Biofilm constructions had been analyzed utilizing a scanning electron microscope (SEM) and confocal microscopy (CM).
Gene expressions of biofilms had been evaluated utilizing actual time RT-PCR. Results of UP publicity on S. pneumoniae colonization to HMEECs had been evaluated utilizing fluorescent in-situ hybridization (FISH), cell viability was detected utilizing the Ezcyto equipment, apoptosis in HMEECs had been evaluated utilizing Annexin-V/PI based mostly cytometry evaluation and reactive oxygen species (ROS) manufacturing had been evaluated utilizing the Oxiselect equipment.
Alteration of HMEECs gene expressions on UP publicity or pneumococci colonization was evaluated utilizing microarray. In vivo colonization of pneumococci within the presence of UP and transition to center ear and lungs had been evaluated utilizing an intranasal mice colonization mannequin. The UP publicity considerably elevated (*p < 0.05) pneumococcal in vitro biofilms and planktonic progress.
Within the presence of UP, pneumococci fashioned organized biofilms with a matrix, whereas in absence of UP micro organism had been unable to type biofilms. The luxS, ply, lytA, comA, comB and ciaR genes concerned in bacterial pathogenesis, biofilm formation and quorum sensing had been up-regulated in pneumococci biofilms grown within the presence of UP.
The HMEECs viability was considerably decreased (p < 0.05) and micro organism colonization was considerably elevated (p < 0.05) in co-treatment (UP + S. pneumoniae) when in comparison with single remedy. Equally, elevated apoptosis and ROS manufacturing had been detected in HMEECs handled with UP + pneumococci.
The microarray evaluation of HMEECs revealed that the genes contain in apoptosis and cell dying, irritation, and immune response, had been up-regulated in co-treatment and had been unchanged or expressed in much less fold in single remedies of UP or S. pneumoniae.
The in vivo research confirmed an elevated pneumococcal colonization of the nasopharynx within the presence of UP and the next transition of micro organism to the center ear and lungs within the presence of UP. The UP publicity elevated S. pneumoniae in vitro biofilm and colonization of HMEECs, and in vivo mouse nasopharyngeal colonization, and elevated dissemination to mouse center ear and lungs.
Oleanolic Acid Mitigates 6-Hydroxydopamine Neurotoxicity by Attenuating Intracellular ROS in PC12 Cells and Striatal Microglial Activation in Rat Brains.
Oleanolic acid (OA), a biologically lively pentacyclic triterpenoid compound, has been implicated in quite a few scientific advantages together with antioxidant, and anti inflammatory properties. OA has been beforehand proven to ameliorate the poisonous results of 6-hydroxydopamine (6-OHDA), nonetheless, the mechanism by which this impact is exhibited just isn’t clearly understood.
Within the current research, we investigated the function of OA in attenuation of microglial activation in 6-OHDA induced Parkinsonian rat mannequin. We additionally explored the flexibility of OA to attenuate 6-OHDA-induced intracellular reactive oxygen species (ROS), and thus forestall cell dying in PC12 cells.
We accessed the utility of immunohistochemistry to evaluate striatal microglial activation, the place form descriptors corresponding to space, perimeter, Feret’s diameter, facet ratio and solidity had been decided utilizing the Fiji ImageJ software program.
Intracellular ROS and cell viability had been assessed in PC12 cells utilizing the OxiSelectTM Intracellular ROS Assay Equipment and MTT assay, respectively. We discovered that microglial activation was decreased in rats pre-treated with OA previous to 6-OHDA insult in addition to in rats handled with OA 1 day publish 6-OHDA publicity when in comparison with untreated rats, as decided by form descriptors.
This discovering was in correlation with considerably improved motor signs and elevated striatal dopamine in handled rats as in comparison with non-treated rats. Circulation cytometry evaluation of PC12 cells revealed a decreased quantity of intracellular ROS in cells pre-treated with OA 6 h previous to 6-OHDA publicity and cells handled with OA 1 h publish 6-OHDA publicity, suggesting that OA gives neuroprotection in PC12 cells by eradicating intracellular ROS, thereby decreasing oxidative stress.
Our discovering recommend that OA displays its neuroprotective impact by attenuating striatal microglial activation, which leads to neuroinflammation that’s implicated in Parkinson’s illness pathology. Additional research detailing the mechanism by which OA interacts with microglia could also be helpful in understanding the function of OA in attenuating neuroinflammation.
Cobalt nanoparticles induce lung damage, DNA injury and mutations in mice.
We and different teams have demonstrated that publicity to cobalt nanoparticles (Nano-Co) prompted oxidative stress and irritation, which have been proven to be strongly related to genotoxic and carcinogenic results. Nonetheless, few research have reported Nano-Co-induced genotoxic results in vivo.
Right here, we suggest that Nano-Co might have excessive genotoxic results as a result of their small dimension and excessive floor space, which have excessive capability for inflicting oxidative stress and irritation.
gpt delta transgenic mice had been used as our in vivo research mannequin. They had been intratracheally instilled with 50 μg per mouse of Nano-Co. At day 1, 3, 7 and 28 after publicity, bronchoalveolar lavage (BAL) was carried out and the variety of neutrophils, CXCL1/KC stage, LDH exercise and focus of whole protein within the BAL fluid (BALF) had been decided.
Mouse lung tissues had been collected for H&E staining, and Ki-67, PCNA and γ-H2AX immunohistochemical staining. 8-OHdG stage within the genomic DNA of mouse lungs was decided by an OxiSelect™ Oxidative DNA Harm ELISA Equipment, and mutant frequency and mutation spectrum within the gpt gene had been additionally decided in mouse lungs at 4 months after Nano-Co publicity by 6-TG choice, colony PCR, and DNA sequencing.
Publicity of mice to Nano-Co (50 μg per mouse) resulted in intensive acute lung irritation and lung damage which had been mirrored by elevated variety of neutrophils, CXCL1/KC stage, LDH exercise and focus of whole protein within the BALF, and infiltration of enormous quantity of neutrophils and macrophages within the alveolar area and interstitial tissues.
Elevated immunostaining of cell proliferation markers, Ki-67 and PCNA, and the DNA injury marker, γ-H2AX, was additionally noticed in bronchiolar epithelial cells and hyperplastic sort II pneumocytes in mouse lungs at day 7 after Nano-Co publicity. At 4 months after publicity, intensive interstitial fibrosis and proliferation of interstitial cells with inflammatory cells infiltrating the alveolar septa had been noticed. Furthermore, Nano-Co prompted elevated stage of 8-OHdG in genomic DNA of mouse lung tissues.
OxiSelect HORAC Activity Assay Kit |
|||
| STA-346-5 | Cell Biolabs | 5 x 192 assays | EUR 2732.4 |
|
Description: The HORAC assay is a powerful tool to measure the antioxidant capacity of biomolecules. Our OxiSelect HORAC Activity Assay measures the degradation of free hydroxyl radicals in less than 2 hours from a wide variety of sample types. |
|||
OxiSelect Comet Assay Control Cells |
|||
| STA-354 | Cell Biolabs | 1 set | EUR 303.6 |
|
Description: OxiSelect Comet Assay Control Cells are a set of two cell lines that serve as a positive control (Etoposide-treated) and a negative control (untreated) respectively. |
|||
OxiSelect Protein Radical ELISA Kit |
|||
| STA-810 | Cell Biolabs | 96 assays | EUR 706.8 |
|
Description: Protein radicals form from electron transfer from various reactive oxygen and reactive nitrogen species. Protein radicals have been linked to various disorders including amyotrophic lateral sclerosis, Huntington's Disease and Alzheimer's disease. The OxiSelect Protein Radical ELISA Kit quantifies free radicals in a standard 96-well plate. |
|||
OxiSelect Glutathione Reductase Assay Kit |
|||
| STA-812 | Cell Biolabs | 100 assays | EUR 505.2 |
|
Description: Cell Biolabs? OxiSelect Glutathione Reductase Assay Kit is a Glutathione reductase activity is defined as 1 unit of enzyme reducing 1 µmole oxidized glutathione (GSSG) per minute at pH 7.6 and 25ºC. The kit employs a simple enzymatic recycling reaction for glutathione quantification where the reduction of a chromagen is correlated to glutathione reductase enzymatic activity. The kit has a detection sensitivity limit of approximately 0.6 mU/mL. Each kit provides sufficient reagents to perform up to 100 assays, including standard curve and unknown samples. |
|||
OxiSelect Polyamine Oxidase Assay Kit |
|||
| XPX-5107 | Cell Biolabs | 100 assays | EUR 540 |
OxiSelect BPDE Protein Adduct ELISA Kit |
|||
| STA-301 | Cell Biolabs | 96 assays | EUR 943.2 |
|
Description: BSA standards or protein samples (10 ?g/mL) are adsorbed onto a 96-well plate for 2 hrs at 37ºC. The BPDE-protein adducts present in the sample or standard are probed with an Anti-BPDE-I Antibody, followed by an HRP Conjugated Secondary Antibody. The BPDE protein adduct content in an unknown sample is determined by comparing with a standard curve that is prepared from predetermined BPDE-BSA standards. |
|||
OxiSelect Nitrotyrosine ELISA Kit (1 plate) |
|||
| STA-305 | Cell Biolabs | 96 assays | EUR 957.6 |
|
Description: Nitric oxide influences a variety of biological processes including cell proliferation, apoptosis, neurotoxicity and extracellular matrix remodeling. Nitric oxide reacts with superoxide to form peroxynitrite, which in turn nitrates tyrosine residues in proteins. Nitrotyrosine therefore serves as a marker for peroxynitrite action in a variety of disease states and in conditions of cellular damage and oxidative stress. Our OxiSelect Nitrotyrosine ELISA Kit provides a sensitive method to measure the formation of 3-nitrotyrosine in proteins. |
|||
OxiSelect Nitrotyrosine ELISA Kit (5 plates) |
|||
| STA-305-5 | Cell Biolabs | 5 x 96 assays | EUR 3888 |
|
Description: Nitric oxide influences a variety of biological processes including cell proliferation, apoptosis, neurotoxicity and extracellular matrix remodeling. Nitric oxide reacts with superoxide to form peroxynitrite, which in turn nitrates tyrosine residues in proteins. Nitrotyrosine therefore serves as a marker for peroxynitrite action in a variety of disease states and in conditions of cellular damage and oxidative stress. Our OxiSelect Nitrotyrosine ELISA Kit provides a sensitive method to measure the formation of 3-nitrotyrosine in proteins. |
|||
OxiSelect Nitrotyrosine ELISA Kit, Trial Size |
|||
| STA-305-T | Cell Biolabs | 32 assays | EUR 505.2 |
|
Description: Nitric oxide influences a variety of biological processes including cell proliferation, apoptosis, neurotoxicity and extracellular matrix remodeling. Nitric oxide reacts with superoxide to form peroxynitrite, which in turn nitrates tyrosine residues in proteins. Nitrotyrosine therefore serves as a marker for peroxynitrite action in a variety of disease states and in conditions of cellular damage and oxidative stress. Our OxiSelect Nitrotyrosine ELISA Kit provides a sensitive method to measure the formation of 3-nitrotyrosine in proteins. |
|||
OxiSelect AOPP Assay Kit (200 assays) |
|||
| STA-318 | Cell Biolabs | 200 assays | EUR 762 |
|
Description: Advanced Oxidation Protein Products (AOPP) are uremic toxins created during oxidative stress through the reaction of plasma proteins with chlorinated oxidants such as chloramines or hypochlorous acid. AOPP levels are elevated in patients with renal complications, diabetes mellitus, and atherosclerosis, as well as HIV-positive patients. Our OxiSelect AOPP Assay Kit provides a convenient method to assess oxidative stress. The kit includes a Chloramine standard and an AOPP Human Serum Albumin conjugate for use as a positive control. |
|||
OxiSelect TBARS Assay Kit (MDA Quantitation) |
|||
| STA-330 | Cell Biolabs | 200 assays | EUR 790.8 |
|
Description: The TBARS (Thiobarbituric Acid Reactive Substances) assay is well-established for screening and monitoring lipid peroxidation. MDA forms a 1:2 adduct with thiobarbituric acid; the MDA-TBA adduct can then be measured. Our OxiSelect TBARS Assay Kit provides a much more user-friendly protocol to measure the MDA-TBA adduct. Reaction volumes are much smaller than the traditional assay, so much less sample is required. Also, reactions can be performed in standard polypropylene tubes - no glass tubes or glass marbles are required. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE. |
|||
OxiSelect TBARS Assay Kit (MDA Quantitation) |
|||
| STA-330-5 | Cell Biolabs | 5 x 200 assays | EUR 3205.2 |
|
Description: The TBARS (Thiobarbituric Acid Reactive Substances) assay is well-established for screening and monitoring lipid peroxidation. MDA forms a 1:2 adduct with thiobarbituric acid; the MDA-TBA adduct can then be measured. Our OxiSelect TBARS Assay Kit provides a much more user-friendly protocol to measure the MDA-TBA adduct. Reaction volumes are much smaller than the traditional assay, so much less sample is required. Also, reactions can be performed in standard polypropylene tubes - no glass tubes or glass marbles are required. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE. |
|||
OxiSelect Catalase Activity Assay Kit, Fluorometric |
|||
| STA-339 | Cell Biolabs | 500 assays | EUR 908.4 |
|
Description: Catalase is a ubiquitous enzyme that destroys hydrogen peroxides formed during oxidative stress. Our OxiSelect Catalase Activity Assay Kit measures catalase activity in less than one hour from a variety of samples including blood, cells and tissues. Each kit provides sufficient reagents to perform up to 500 assays in a 96-well microtiter plate. This includes blanks, catalase standards and unknown samples. The OxiSelect Catalase Activity Assay Kit (Fluorometric) provides a 40-fold increase in sensitivity compared to our colorimetric assay. |
|||
OxiSelect Superoxide Dismutase Activity Assay Kit |
|||
| STA-340 | Cell Biolabs | 100 assays | EUR 762 |
|
Description: Superoxide dismutase (SOD), which catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen, is one of the most important antioxidant enzymes. SOD enzymes are classified into three groups: cytosolic CuZn-SOD, mitochondrial Mn-SOD, and extracellular Ec-SOD. Our OxiSelect Superoxide Dismutase Activity Assay uses a xanthine/xanthine oxidase (XOD) system to generate superoxide anions and a chromagen to produce a water-soluble formazan dye upon reduction by superoxide anions. The superoxide dismutase activity is determined as the inhibition or reduction of chromagen. |
|||
OxiSelect Catalase Activity Assay Kit (Colorimetric) |
|||
| STA-341 | Cell Biolabs | 96 assays | EUR 811.2 |
|
Description: Catalase is a ubiquitous enzyme that destroys hydrogen peroxides formed during oxidative stress. Our OxiSelect Catalase Activity Assay Kits measure catalase activity in less than one hour from a variety of samples including blood, cells and tissues. Each kit provides sufficient reagents to perform up to 100 assays in a 96-well microtiter plate, or 50 assays in microcentrifuge tubes. This includes blanks, catalase standards and unknown samples. Direct spectrophotometric detection of catalase activity with ultraviolet light can cause interference from proteins and other biological components. The OxiSelect Catalase Activity Assay Kit (Colorimetric) utilizes visible light (520 nm), which reduces sample interference. The OxiSelect Catalase Activity Assay Kit (Fluorometric) provides a 40-fold increase in sensitivity compared to our colorimetric assay. |
|||
OxiSelect Comet Assay Slides (3-Well) |
|||
| STA-352 | Cell Biolabs | 5 slides | EUR 261.6 |
|
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy. |
|||
OxiSelect Comet Assay Slides (3-Well) |
|||
| STA-353 | Cell Biolabs | 25 slides | EUR 435.6 |
|
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy. |
|||
OxiSelect Comet Assay Slides (3-Well) |
|||
| STA-353-5 | Cell Biolabs | 5 x 25 slides | EUR 1521.6 |
|
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy. |
|||
OxiSelect 96-Well Comet Assay Kit |
|||
| STA-355 | Cell Biolabs | 96 assays | EUR 512.4 |
|
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope. |
|||
OxiSelect 96-Well Comet Assay Kit |
|||
| STA-355-5 | Cell Biolabs | 5 x 96 assays | EUR 1959.6 |
|
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope. |
|||
OxiSelect 96-Well Comet Assay Slide |
|||
| STA-356 | Cell Biolabs | 1 slide | EUR 219.6 |
|
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy. |
|||
OxiSelect 96-Well Comet Assay Slide |
|||
| STA-356-5 | Cell Biolabs | 5 slides | EUR 622.8 |
|
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy. |
|||
OxiSelect BPDE DNA Adduct ELISA Kit |
|||
| STA-357 | Cell Biolabs | 96 assays | EUR 908.4 |
|
Description: BPDE-DNA standards or unknown DNA samples are adsorbed onto a 96-well DNA high-binding plate. The BPDE-DNA adducts present in the sample or standard are probed with an Anti-BPDE-I Antibody, followed by an HRP Conjugated Secondary Antibody. The BPDE-DNA adduct content in an unknown sample is determined by comparing with a standard curve that is prepared from predetermined BPDE-DNA standards. |
|||
OxiSelect Methylglyoxal (MG) Competitive ELISA Kit |
|||
| STA-811 | Cell Biolabs | 96 assays | EUR 957.6 |
|
Description: The OxiSelect Methylglyoxal (MG) ELISA Kit is an enzyme immunoassay developed for rapid detection and quantitation of MG-H1 (methyl-glyoxal-hydro-imidazolone) protein adducts. The quantity of MG adduct in protein samples is determined by comparing its absorbance with that of a known MG-BSA standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples. |
|||
OxiSelect Methylglyoxal (MG) Competitive ELISA Kit |
|||
| STA-811-5 | Cell Biolabs | 5 x 96 assays | EUR 3888 |
|
Description: The OxiSelect Methylglyoxal (MG) ELISA Kit is an enzyme immunoassay developed for rapid detection and quantitation of MG-H1 (methyl-glyoxal-hydro-imidazolone) protein adducts. The quantity of MG adduct in protein samples is determined by comparing its absorbance with that of a known MG-BSA standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples. |
|||
OxiSelect MDA Adduct Competitive ELISA Kit |
|||
| STA-832 | Cell Biolabs | 96 assays | EUR 957.6 |
|
Description: MDA, or malondialdehyde, is a widely accepted marker for oxidative stress. Our OxiSelect MDA Competitive ELISA Kit provides a sensitive, specific method for detection of this lipid peroxidation by-product. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE. |
|||
OxiSelect MDA Adduct Competitive ELISA Kit |
|||
| STA-832-5 | Cell Biolabs | 5 x 96 assays | EUR 3888 |
|
Description: MDA, or malondialdehyde, is a widely accepted marker for oxidative stress. Our OxiSelect MDA Competitive ELISA Kit provides a sensitive, specific method for detection of this lipid peroxidation by-product. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE. |
|||
OxiSelect HNE Adduct Competitive ELISA Kit |
|||
| STA-838 | Cell Biolabs | 96 assays | EUR 957.6 |
|
Description: 4-hydroxynonenal (4-HNE or HNE) is a well known by-product of lipid peroxidation and is widely accepted as a stable marker for oxidative stress. Our OxiSelect HNE Adduct Competitive ELISA Kit measures the formation of HNE adducts to any protein residue using a competitive ELISA format. |
|||
OxiSelect HNE Adduct Competitive ELISA Kit |
|||
| STA-838-5 | Cell Biolabs | 5 x 96 assays | EUR 3888 |
|
Description: 4-hydroxynonenal (4-HNE or HNE) is a well known by-product of lipid peroxidation and is widely accepted as a stable marker for oxidative stress. Our OxiSelect HNE Adduct Competitive ELISA Kit measures the formation of HNE adducts to any protein residue using a competitive ELISA format. |
|||
OxiSelect Ascorbic Acid Assay Kit (FRASC) |
|||
| STA-860 | Cell Biolabs | 200 assays | EUR 672 |
|
Description: Ascorbic acid is a vital water-soluble antioxidant found in living organisms. Ascorbic acid is critical for a variety of functions related to tissue growth and wound healing, neurotransmitter formation, blood cholesterol levels, as well as free radical neutralization. The OxiSelect Ascorbic Acid Assay Kit is a quantitative assay for measuring ascorbic acid within various samples. The assay is based on the Ferric Reducing/Antioxidant Ascorbic Acid (FRASC) chemistry driven by the electron donating reducing power of antioxidants. The kit employs ascorbate oxidase, which allows the user to differentiate the ascorbic acid content from other antioxidants present within their sample. Ascorbic acid levels in a sample are determined by measuring the difference in optical density between two sample wells, one with and one without the enzyme. |
|||
OxiSelect Protein Carbamylation Sandwich ELISA Kit |
|||
| STA-877 | Cell Biolabs | 96 assays | EUR 936 |
|
Description: The OxiSelect Protein Carbamylation Sandwich ELISA Kit is an enzyme immunoassay developed for rapid detection and quantitation of protein carbamylation. The quantity of carbamylated adduct in protein samples is determined by comparing its absorbance with that of a known CBL-BSA standard curve. The kit has a detection sensitivity limit of 1.5 ng/mL of CBL-BSA. |
|||
OxiSelect Monoamine Oxidase Assay Kit (Fluorometric) |
|||
| XPX-5000 | Cell Biolabs | 96 assays | EUR 540 |
|
Description: Our Monoamine Oxidase Assay Kit measures MOA-A and MAO-B in biological samples. MAO reacts with a substrate, generating hydrogen peroxide. A reaction between hydrogen peroxide and the probe results in a signal that is directly proportional to the level of MAO in the sample and is detected fluorescence plate reader. MAO activity in unknown samples is calculated based on a hydrogen peroxide standard curve. |
|||
OxiSelect Monoamine Oxidase Assay Kit (Fluorometric) |
|||
| XPX-5000-5 | Cell Biolabs | 5 x 96 assays | EUR 2154 |
|
Description: Our Monoamine Oxidase Assay Kit measures MOA-A and MAO-B in biological samples. MAO reacts with a substrate, generating hydrogen peroxide. A reaction between hydrogen peroxide and the probe results in a signal that is directly proportional to the level of MAO in the sample and is detected fluorescence plate reader. MAO activity in unknown samples is calculated based on a hydrogen peroxide standard curve. |
|||
OxiSelect Monoamine Oxidase Assay Kit (Colorimetric) |
|||
| XPX-5006 | Cell Biolabs | 96 assays | EUR 540 |
|
Description: Our Monoamine Oxidase Assay Kit measures MOA-A and MAO-B in biological samples. MAO reacts with a substrate, generating hydrogen peroxide. A reaction between hydrogen peroxide and the probe results in a signal that is directly proportional to the level of MAO in the sample and is detected colorimetric plate reader. MAO activity in unknown samples is calculated based on a hydrogen peroxide standard curve. |
|||
OxiSelect Monoamine Oxidase Assay Kit (Colorimetric) |
|||
| XPX-5006-5 | Cell Biolabs | 5 x 96 assays | EUR 2154 |
|
Description: Our Monoamine Oxidase Assay Kit measures MOA-A and MAO-B in biological samples. MAO reacts with a substrate, generating hydrogen peroxide. A reaction between hydrogen peroxide and the probe results in a signal that is directly proportional to the level of MAO in the sample and is detected colorimetric plate reader. MAO activity in unknown samples is calculated based on a hydrogen peroxide standard curve. |
|||
OxiSelect Protein Carbonyl Fluorometric Assay, Trial Size |
|||
| STA-307-T | Cell Biolabs | 20 assays | EUR 421.2 |
|
Description: The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, lysine, arginine and threonine residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species. Our OxiSelect Protein Carbonyl Fluorometric Assay Kit provides a rapid, efficient method for the detection of protein carbonyl residues. The fluorescence plate-based format provides a convenient system for direct measurement of protein carbonyl content. |
|||
OxiSelect Protein Carbonyl Immunoblot Kit, Trial Size |
|||
| STA-308-T | Cell Biolabs | 4 blots | EUR 316.8 |
|
Description: The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, lysine, arginine and threonine residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species. Our OxiSelect Protein Carbonyl Immunoblot Kit provides a rapid, efficient method for the detection of protein carbonyl residues. The immunoblot format provides a convenient way to compare oxidized and non-oxidized protein fingerprints. |
|||
OxiSelect Protein Carbonyl ELISA Kit (1 plate) |
|||
| STA-310 | Cell Biolabs | 96 assays | EUR 957.6 |
|
Description: The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, lysine, arginine and threonine residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species. Our OxiSelect Protein Carbonyl ELISA Kit provides a rapid, efficient method for the detection of protein carbonyl residues. The ELISA format is perfect for higher throughput and high sensitivity; we have eliminated concentration and precipitation steps allowing greater sample retention. |
|||
OxiSelect Protein Carbonyl ELISA Kit (5 plates) |
|||
| STA-310-5 | Cell Biolabs | 5 x 96 assays | EUR 3888 |
|
Description: The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, lysine, arginine and threonine residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species. Our OxiSelect Protein Carbonyl ELISA Kit provides a rapid, efficient method for the detection of protein carbonyl residues. The ELISA format is perfect for higher throughput and high sensitivity; we have eliminated concentration and precipitation steps allowing greater sample retention. |
|||
Nano-Co additionally induced a a lot larger mutant frequency as in comparison with controls, and the commonest mutation was G:C to T:A transversion, which can be defined by Nano-Co-induced elevated formation of 8-OHdG.
Our research demonstrated that publicity to Nano-Co prompted oxidative stress, lung irritation and damage, and cell proliferation, which additional resulted in DNA injury and DNA mutation. These findings have vital implications for understanding the potential well being results of nanoparticle publicity.
