Haemonchus contortus is without doubt one of the most pathogenic and economically essential parasites of sheep. Completely different H. contortus antigens; crude somatic antigen (CSA), excretory/secretory antigen (ESA), crude larval antigen (CLA), glutathione-S-transferase antigen (GST) and recombinant protein (rhcp 26/23) had been ready and characterised utilizing sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS–PAGE) and western blot.
The antigens had been immunologically evaluated by way of oblique enzyme linked immunosorbent assay (ELISA) for prognosis of haemonchosis in experimentally and naturally contaminated sheep. Evaluation of the resultant bands of SDS–PAGE demonstrated that 13, 6, 11, 2 and 1 protein bands from CSA, ESA, CLA, GST and rhcp 26/23, respectively and evaluation of the resultant bands of western blot confirmed that 13, 6, Four and 1 reactive bands detected from CSA, ESA, CLA and GST, respectively.
The outcomes of ELISA of various antigens revealed that sero-prevelance of CSA, ESA, CLA, GST and rhcp 26/23 had been 78.51, 82.34, 85.319, 45.319 and 90.8% respectively, sensitivity had been 100, 90, 100, 96.66 and 90%, respectively and specificity had been 0, 70, 10, 70 and 6.66%, respectively with diagnostic efficiency had been 50, 80, 55, 83.33 and 48.33%, respectively. Statistical evaluation utilizing Chi sq. take a look at discovered that GST is the very best one which can be utilized.
Finegel SDS-PAGE Running Buffer(10X) |
F1504-050 |
GenDepot |
500ml |
EUR 181.2 |
Finegel SDS-PAGE Running Buffer(10X) |
F1504-100 |
GenDepot |
2x500ml |
EUR 241.2 |
SDS-PAGE Resolving Gel Casting Solution |
P9050-050 |
GenDepot |
500ml |
EUR 105.6 |
SDS-PAGE Resolving Gel Casting Solution |
P9050-100 |
GenDepot |
2X500ml |
EUR 144 |
SDS-PAGE Stacking Gel Casting Solution |
P9051-010 |
GenDepot |
100ml |
EUR 96 |
SDS-PAGE Stacking Gel Casting Solution |
P9051-050 |
GenDepot |
500ml |
EUR 134.4 |
Western Blot Enhancing Kit |
K3172120 |
Biochain |
1200 cm2 |
EUR 171.6 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
Western Blot Enhancing Kit |
K3172250 |
Biochain |
2500 cm2 |
EUR 225.6 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
Middle range pre-stained (blue) protein markers (19-110 kda) for Western (SDS-PAGE) |
MMWM-14 |
Alpha Diagnostics |
200 ul |
EUR 196.8 |
SDS-PAGE Protein Loading Buffer 2X (Reducing) |
AR0131 |
BosterBio |
6mL |
EUR 72 |
SDS-PAGE Protein Loading Buffer 5X (Reducing) |
AR1112 |
BosterBio |
3mL |
EUR 72 |
Finegel SDS-PAGE Running Gel Solution(10%) |
F1500-100 |
GenDepot |
2x500ml |
EUR 225.6 |
Finegel SDS-PAGE Running Gel Solution(8%) |
F1501-100 |
GenDepot |
2X500ml |
EUR 225.6 |
Finegel SDS-PAGE Running Gel Solution(12.5%) |
F1502-100 |
GenDepot |
2X500ml |
EUR 225.6 |
Finegel SDS-PAGE Running Gel Solution(15%) |
F1503-100 |
GenDepot |
2X500ml |
EUR 225.6 |
Xpert SDS-PAGE Gel Running Buffer(10X) |
P9000-050 |
GenDepot |
500ml |
EUR 116.4 |
Xpert SDS-PAGE Gel Running Buffer(10X) |
P9000-100 |
GenDepot |
1L |
EUR 165.6 |
Interferon alpha Western Blot Antibody |
20-abx137014 |
Abbexa |
|
|
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PDCD4 Western Blot kit (AWBK09045) |
AWBK09045 |
Aviva Systems Biology |
10 reactions |
EUR 776.4 |
Description: - Description of target:
- Species reactivity:
- Application:
- Assay info:
- Sensitivity:
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ETS1 Western Blot kit (AWBK100604) |
AWBK100604 |
Aviva Systems Biology |
10 reactions |
EUR 776.4 |
Description: - Description of target:
- Species reactivity:
- Application:
- Assay info:
- Sensitivity:
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ERG Western Blot kit (AWBK100625) |
AWBK100625 |
Aviva Systems Biology |
10 reactions |
EUR 776.4 |
Description: - Description of target:
- Species reactivity:
- Application:
- Assay info:
- Sensitivity:
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GABRP Western Blot kit (AWBK13034) |
AWBK13034 |
Aviva Systems Biology |
10 reactions |
EUR 776.4 |
Description: - Description of target:
- Species reactivity:
- Application:
- Assay info:
- Sensitivity:
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HTR1A Western Blot kit (AWBK13041) |
AWBK13041 |
Aviva Systems Biology |
10 reactions |
EUR 776.4 |
Description: - Description of target:
- Species reactivity:
- Application:
- Assay info:
- Sensitivity:
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The cross reactivity of GST antigen, crude Fasciola antigen and crude Moniezia antigen examined versus their homologous hyper immune sera at totally different dilutions utilizing ELISA. The present research reported that GST antigen might be thought of as a promising antigen for prognosis of haemonchosis.