Haemonchus contortus is without doubt one of the most pathogenic and economically essential parasites of sheep. Completely different H. contortus antigens; crude somatic antigen (CSA), excretory/secretory antigen (ESA), crude larval antigen (CLA), glutathione-S-transferase antigen (GST) and recombinant protein (rhcp 26/23) had been ready and characterised utilizing sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS–PAGE) and western blot.
The antigens had been immunologically evaluated by way of oblique enzyme linked immunosorbent assay (ELISA) for prognosis of haemonchosis in experimentally and naturally contaminated sheep. Evaluation of the resultant bands of SDS–PAGE demonstrated that 13, 6, 11, 2 and 1 protein bands from CSA, ESA, CLA, GST and rhcp 26/23, respectively and evaluation of the resultant bands of western blot confirmed that 13, 6, Four and 1 reactive bands detected from CSA, ESA, CLA and GST, respectively.
The outcomes of ELISA of various antigens revealed that sero-prevelance of CSA, ESA, CLA, GST and rhcp 26/23 had been 78.51, 82.34, 85.319, 45.319 and 90.8% respectively, sensitivity had been 100, 90, 100, 96.66 and 90%, respectively and specificity had been 0, 70, 10, 70 and 6.66%, respectively with diagnostic efficiency had been 50, 80, 55, 83.33 and 48.33%, respectively. Statistical evaluation utilizing Chi sq. take a look at discovered that GST is the very best one which can be utilized.
Finegel SDS-PAGE Running Buffer(10X) |
F1504-100 |
GenDepot |
2x500ml |
EUR 201 |
SDS-PAGE Resolving Gel Casting Solution |
P9050-050 |
GenDepot |
500ml |
EUR 88 |
SDS-PAGE Resolving Gel Casting Solution |
P9050-100 |
GenDepot |
2X500ml |
EUR 120 |
SDS-PAGE Stacking Gel Casting Solution |
P9051-010 |
GenDepot |
100ml |
EUR 80 |
SDS-PAGE Stacking Gel Casting Solution |
P9051-050 |
GenDepot |
500ml |
EUR 112 |
Western Blot Enhancing Kit |
K3172120 |
Biochain |
1200 cm2 |
EUR 143 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
Western Blot Enhancing Kit |
K3172250 |
Biochain |
2500 cm2 |
EUR 188 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
SDS-PAGE Protein Loading Buffer 2X (Reducing) |
AR0131 |
BosterBio |
6mL |
EUR 60 |
SDS-PAGE Protein Loading Buffer 5X (Reducing) |
AR1112 |
BosterBio |
3mL |
EUR 60 |
Finegel SDS-PAGE Running Gel Solution(10%) |
F1500-100 |
GenDepot |
2x500ml |
EUR 188 |
Finegel SDS-PAGE Running Gel Solution(8%) |
F1501-100 |
GenDepot |
2X500ml |
EUR 188 |
Finegel SDS-PAGE Running Gel Solution(12.5%) |
F1502-100 |
GenDepot |
2X500ml |
EUR 188 |
Finegel SDS-PAGE Running Gel Solution(15%) |
F1503-100 |
GenDepot |
2X500ml |
EUR 188 |
Xpert SDS-PAGE Gel Running Buffer(10X) |
P9000-050 |
GenDepot |
500ml |
EUR 97 |
Xpert SDS-PAGE Gel Running Buffer(10X) |
P9000-100 |
GenDepot |
1L |
EUR 138 |
Human Versican (VS) ELISA Kit |
DLR-VS-Hu-48T |
DL Develop |
48T |
EUR 498 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Versican (VS) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Human Versican (VS) ELISA Kit |
DLR-VS-Hu-96T |
DL Develop |
96T |
EUR 647 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Versican (VS) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Mouse Versican (VS) ELISA Kit |
DLR-VS-Mu-48T |
DL Develop |
48T |
EUR 508 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Versican (VS) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Mouse Versican (VS) ELISA Kit |
DLR-VS-Mu-96T |
DL Develop |
96T |
EUR 661 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Versican (VS) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Human Versican (VS) ELISA Kit |
RD-VS-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 500 |
Human Versican (VS) ELISA Kit |
RD-VS-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 692 |
Mouse Versican (VS) ELISA Kit |
RD-VS-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 511 |
Mouse Versican (VS) ELISA Kit |
RD-VS-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 709 |
Human Versican (VS) ELISA Kit |
RDR-VS-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 522 |
The cross reactivity of GST antigen, crude Fasciola antigen and crude Moniezia antigen examined versus their homologous hyper immune sera at totally different dilutions utilizing ELISA. The present research reported that GST antigen might be thought of as a promising antigen for prognosis of haemonchosis.