Such novel assay may even give out the self-assembly kinetic fixed of QDs and YPYDH6 as KD of 34.1 μM with n (binding cooperativeness) of two.2 by Hill equation. Extra importantly, the simultaneous detection of the meeting and imidazole induced disassembly of the QD-YPYDH6 -anti-HA complicated was achieved in a single in-capillary assay. Our research demonstrated a brand new technique for the web detection of antigen-antibody interactions.
Protein microarray system for detecting protein-protein interactions utilizing an anti–His–tag antibody and fluorescence scanning: results of the heme redox state on protein-protein interactions of heme-regulated phosphodiesterase from Escherichia coli.
A extremely delicate microarray system for detecting protein-protein interactions has been developed. This technique was efficiently utilized to investigate the interactions of heme-regulated phosphodiesterase from Escherichia coli (Ec DOS). To immobilize 6-Tag fused Ec DOS, anti-(His)6-Tag monoclonal antibody was initially immobilized on the strong floor, and (His)6-Tag fused Ec DOS was mounted by antigen-antibody interactions.
For this experiment, ProteoChip, typically appropriate for antibody immobilization, was used as strong substrate. On this report, we affirm the antibody immobilization skill of ProteoChip and particular binding to the F(c) area of the antibody. Based mostly on this discovering, interdomain interactions between Ec DOS and the remoted heme-bound PAS area had been investigated on the strong floor.
Ec DOS immobilized by way of anti-6-Tag mAb maintained interactions with the PAS fragment, in distinction to straight immobilized Ec DOS within the absence of anti-(His)6-Tag mAb. Heme-redox-sensitive interactions between Ec DOS and the PAS fragment had been moreover detected utilizing anti-(His)6-Tag mAb as a mediator.
Our outcomes collectively recommend that the immobilization technique utilizing anti-Tag antibody is appropriate for sustaining native protein traits to facilitate elucidation of their buildings and features on strong surfaces.
Enhancement of scFv fragment reactivity with goal antigens in binding assays following mixing with anti–tag monoclonal antibodies.
The phage show Ab library know-how has been discovered to be a helpful technique to isolate antigen-specific Ab fragments, for the reason that repertoire of antibody specificities is broad and because it bypasses the necessity of immunization. Nonetheless, when screening clones remoted from a phage show Ab library, the yield of isolating antigen-specific Ab fragments is low and the speed of false adverse outcomes is excessive.
This limitation displays the low affinity/avidity of Ab fragments and/or the low density of the goal antigen. To facilitate the isolation of Ab fragments with a broad vary of affinities to antigens of curiosity from phage show Ab libraries, we now have developed a easy technique to extend the sensitivity of binding assays to detect the reactivity of single-chain fragments of antibody variable areas (scFv) with goal antigens.
This technique includes the blending of scFv fragments, expressing a c-myc epitope tag, with anti-tag mAb 9E10 previous to their use in binding assays as a way to kind secure dimeric Ab fragment-anti-tag mAb complexes. The rise within the reactivity of scFv fragments with the corresponding antigen is noticed over a broad vary of scFv fragment (6-800 microg/ml) and mAb 9E10 concentrations, thereby facilitating the testing of scFv fragment preparations with unknown scFv fragment concentrations.
Anti-His-Tag antibody |
STJ97043 |
St John's Laboratory |
200 µl |
EUR 236.4 |
Description: Mouse monoclonal to His-Tag. |
Anti-His-Tag antibody |
STJ97079 |
St John's Laboratory |
200 µl |
EUR 236.4 |
Description: Rabbit polyclonal to His-Tag. |
Anti-His-tag antibody |
STJ99289 |
St John's Laboratory |
200 µl |
EUR 236.4 |
Description: Mouse monoclonal to His-tag. |
Histidine-tag (poly-His/Hisx6) Protein (His-tag-fusion protein) ELISA Kit, 96 tests, Quantitative |
800-440-HIS |
Alpha Diagnostics |
1 kit |
EUR 1074 |
anti-His-Tag |
LF-MA20209 |
Abfrontier |
50 ul |
EUR 400.8 |
Description: Mouse monoclonal to His-Tag |
anti-His-Tag |
LF-PA20210 |
Abfrontier |
100 ul |
EUR 400.8 |
Description: Rabbit polyclonal to His-Tag |
anti- 6*His, His-Tag antibody |
FNab00008 |
FN Test |
100µg |
EUR 606.3 |
|
Description: Antibody raised against 6*His, His-Tag |
anti- 6*His, His-Tag antibody |
FNab00009 |
FN Test |
100µg |
EUR 606.3 |
|
Description: Antibody raised against 6*His, His-Tag |
anti- 6*his,His tag antibody |
FNab00010 |
FN Test |
100µg |
EUR 606.3 |
|
Description: Antibody raised against 6*his,His tag |
Anti-His-tag antibody (KLH) |
STJ90106 |
St John's Laboratory |
200 µl |
EUR 236.4 |
Description: Rabbit polyclonal to His-tag. |
anti-His-Tag (M1) |
LF-MA20419 |
Abfrontier |
100 ug |
EUR 400.8 |
Description: Mouse monoclonal to His Tag |
Histidine Tag (His-Tag) Antibody |
abx412016-01mg |
Abbexa |
0.1 mg |
EUR 594 |
|
Histidine Tag (His-Tag) Antibody |
abx413027-1mg |
Abbexa |
1 mg |
EUR 978 |
|
Histidine Tag (His-Tag) Antibody |
abx413031-2mg |
Abbexa |
2 mg |
EUR 1779.6 |
|
Histidine Tag (His-Tag) Antibody |
abx413032-01mg |
Abbexa |
0.1 mg |
EUR 493.2 |
|
Mouse Anti-His Tag Monoclonal Antibody |
AKR-003 |
Cell Biolabs |
100 µg |
EUR 609.6 |
Description: Mouse Anti-His Tag Monoclonal Antibody is Protein A purified and provided at 1 mg/mL. Suitable for Western blot or Immunostaining. 100 µg. |
Anti- His-Tag Rabbit Monoclonal Antibody |
A1138-50 |
Biovision |
|
EUR 444 |
Anti-His Tag Antibody (monoclonal, 9C11) |
M30975 |
BosterBio |
100ul/vial |
EUR 476.4 |
Description: Mouse IgG monoclonal antibody for His Tag detection. Tested with WB, Dot, ELISA in all species. |
Anti-His Tag Antibody (monoclonal, 19b10) |
M30975-1 |
BosterBio |
100ug/vial |
EUR 352.8 |
His-tag Antibody |
1-CSB-PA000086 |
Cusabio |
|
|
|
Description: A polyclonal antibody against His-tag. Recognizes His-tag from . This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/1000-1/3000.ELISA:1/20000 |
His-Tag Antibody |
1-CSB-PA000340 |
Cusabio |
|
|
|
Description: A polyclonal antibody against His-Tag. Recognizes His-Tag from . This antibody is Unconjugated. Tested in the following application: WB;WB:1:5000 |
His Tag antibody |
70R-41556 |
Fitzgerald |
100 ug |
EUR 523.2 |
Description: Purified Rabbit polyclonal His Tag antibody |
His Tag antibody |
70R-HR005 |
Fitzgerald |
100 ug |
EUR 457.2 |
Description: Affinity purified Rabbit polyclonal His Tag antibody |
Use of this technique in binding assays resulted in a twofold enhance within the reactivity of low-affinity purified scFv fragments with the corresponding antigen. Furthermore, utility of this technique to display screen clones remoted from phage show scFv libraries resulted in a reproducible enhance in each the yield of antigen-specific scFv clones and the titer of scFv fragment preparations by an element of 5 and 2- to 32-fold, respectively.