What’s Inside The PCT Start Kit?
Are you interested in tissue culture but not sure where to start? The PCT Starter Kit is a PERFECT opportunity for you to test and refine your new skill set!
In this package you will receive:
- 3 x 250 ml glass tissue culture vessels
- 100 g of Agar powder
- 5L of MS Media Powder
- 10ml PPM
About 250ML Glass Tissue Growing Cups:
Description: 12x250ml thickened glass culture vessel. Sterilizable in autoclave.
Feature: heat resistant
Shape: a cylindrical container
Material: borosilicate glass 3.3
Width: 2.60 In
Height: 3.50 in.
Diameter: 2.60 in.
About Agar Powder:
Polysaccharide complex obtained by bleaching and extraction with hot water of astrocytes from the red algae Rhodophyceae. The typical usage rate of 6 to 12 g / L of medium. Supreme Agar offers greater clarity for plant growing media.
1050 GM / CM ^ 2
100g, 500g, 1Kg in polyethene bottles
Agar is used as a solid microbial culture medium. The substance is often used in doses ranging from 0.5 to 1.0% (w / v) to solidify plant tissue culture media. Agar is an essential ingredient used for in vitro root development.
Instructions for use:
Dissolve 4.54gms of the dehydrated medium in 600ml of distilled or deionized water at room temperature (15-30 * C). Rinse the vial of medium with a small amount of distilled water to remove traces of energy. Add desired thermoset supplements before autoclaving. Continue stirring until the powder has dissolved. Sometimes the media does not dissolve completely unless the pH is lowered. For these, lower the pH to about 3.0 to facilitate the dissolution of the medium.
The pH of the medium is adjusted using 1N HCl / 1N NaOH / 1N KOH. Make up the final volume to 1000 ml with distilled water. Mix gently, heat, and rotate between intervals until the solution turns clear. Do not boil, reheat and allow to cool below 50 * C during dispensing. Dispense the medium into suitable containers, cap or cap, then autoclave at 15pso (121 ° C) for 15 minutes, using a slow extraction cycle. Higher temperatures and/or longer times are not recommended. Cool autoclaved culture vessels containing medium to 45-50 ° C and aseptically add the desired heat-labile sterile substrate.
Note: The media should be prepared according to the formula mentioned on the label, however, it is recommended to use a full container at a time. Heat resistant substrates should be added after autoclaving.
Storage and shelf life:
Dehydrated plant tissue culture media are hygroscopic and must be protected from sunlight and moisture. Store prepared medium at 2-8 * C protected from direct light. The medium must be used before the expiration date. The full volume of each bottle should be used immediately after opening or the unused portion should be stored in desiccators and refrigerated at 2-8 ° C.
About The Plant Conservative Mixture (PPM):
PPM ™ is a thermostable preservative/biocide that can be used to effectively prevent or reduce microbial contamination in plant tissue culture
In optimal dosages, PPM ™, which stands for Plant Preservative Mixture ™, is an extremely effective preservative/biocide but does not alter in vitro seed germination, callus proliferation and callus regeneration.
PPM ™ prevents bacterial and fungal spore germination. It is heat stable and therefore can be autoclaved with the medium.
PPM ™ can and should be used as a standard ingredient in plant tissue culture media, and it is also substantially less expensive than commonly used antibiotics. Despite the stricter use of sterile techniques, contamination of plant cell and tissue cultures remains a persistent problem that can result in losses ranging from a small number of cultures to the loss of entire batches.
While PPM ™ was primarily designed to inhibit air pollution, water pollution, and pollution introduced by human contact, it can also, in many cases, be used to reduce endogenous pollution.