The ChromaFlash ™ Plant Chromatin Extraction Kit is a complete set of buffers and reagents optimized for isolating plant chromatin or DNA-protein complexes in a simple and fast format. Chromatin prepared with this kit can be used in a variety of chromatin immunoprecipitation methods. It is also the recommended method for obtaining the chromatin required by the Epigentek One-Hour ChIP method using the ChromaFlash ™ One-Step ChIP Kit or the ChromaFlash ™ One-Step Magnetic ChIP Kit. Isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays or nuclear enzyme assays.
Extremely fast procedure: the entire procedure, from plant samples to ready-to-use chromatin, takes less than 2 hours.
Convenient and Flexible: The kit is suitable for preparing both native chromatin and cross-linked chromatin from monolayer or suspension cells, or tissues.
The uncut chromatin makes it customizable for various analysis workflows that require intact or fragmented chromatin, including chip, in vitro protein-DNA interaction analysis, nuclear enzyme assays, and more.
Chromatin immunoprecipitation (ChIP) offers an advantageous tool for studying protein-DNA interaction. With ChIP, the experimenter can determine whether a specific protein binds to specific sequences of a gene in living cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip) or sequencing (ChIP-Seq) techniques.
For example, measurement of the amount of histone H3 methylated in lysine 9 (meH3-K9) associated with a promoter region of a specific gene under various conditions can be achieved by a ChIP-PCR assay, while recruitment of meH3 -K9 to promoters on the ChIP-chip can detect a whole genome-scale. In particular, there is a great demand for a ChIP method with specific antibodies directed against various transcription factors.
Principle and procedure
The ChromaFlash ™ Plant Chromatin Extraction Kit contains all the reagents necessary for successful chromatin extraction directly from plants. The sample cell membranes, with or without crosslinking, are decomposed using the provided lysis buffer. The chromatin or DNA-protein complex is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and stored at the appropriate temperature.