Rapid diagnosis of Hemophilus influenzae type b infections by latex particle agglutination and counterimmunoelectrophoresis.

Rapid diagnosis of Hemophilus influenzae type b infections by latex particle agglutination and counterimmunoelectrophoresis. post thumbnail image
Latex particle agglutination and counterimmunoelectrophoresis strategies have been in contrast in a scientific trial to display their sensitivity, specificity, and usefulness within the fast analysis of invasive Hemophilus influenzae kind b illness. LPA, a simplified LPA carried out by home officers, and CIE used on this research detected 0.2, 0.5, and 1 to 10 ng/ml of Hib capsular antigen, respectively. 100-six sicknesses suspected of being brought on by Hib have been evaluated prospectively by these assays.
A complete of 39 of those have been confirmed by tradition or detection of antigen or each. LPA and simplified LPA have been extra delicate and particular than CIE within the analysis of Hib illness (P lower than 0.01), particularly in invasive illness aside from meningitis.
LPA is cheap, may be carried out rapidly, and detects all invasive Hib infections. The outcomes emphasize the usefulness of antigen detection within the fast analysis of Hib infections, display that LPA is extra delicate and particular than CIE, and may be conveniently carried out by physicians.

Improved detection of nasopharyngeal cocolonization by a number of pneumococcal serotypes by use of latex agglutination or molecular serotyping by microarray.

Identification of Streptococcus pneumoniae within the nasopharynx is important for an understanding of transmission, estimates of vaccine efficacy, and potential alternative illness. Typical nasopharyngeal swab (NPS) tradition and serotyping (the WHO protocol) is prone to underestimate multiple-serotype carriage. We in contrast the WHO protocol with strategies aimed toward enhancing cocolonization detection. 100 twenty-five NPSs from an toddler pneumococcal-carriage research, containing ≥ 1 serotype by WHO tradition, have been recultured in duplicate.
A sweep of colonies from one plate tradition was serotyped by latex agglutination. DNA extracted from the second plate was analyzed by S. pneumoniae molecular-serotyping microarray. A number of serotypes have been detected in 11.2% of the swabs by WHO tradition, 43.2% by sweep serotyping, and 48.8% by microarray. Sweep and microarray have been extra prone to detect a number of serotypes than WHO tradition (P < 0.0001).
Cocolonization detection charges have been related between microarray and sweep, however the microarray recognized the best variety of serotypes. A standard serogroup kind was recognized in 95.2% of swabs by all strategies. WHO methodology considerably underestimates multiple-serotype carriage in comparison with these alternate strategies. Sweep serotyping is cost-effective and discipline deployable however might fail to detect serotypes at low abundance, whereas microarray serotyping is extra pricey and expertise dependent however might detect these extra minor carried serotypes.
latex agglutination, Rapid diagnosis of Hemophilus influenzae type b infections by latex particle agglutination and counterimmunoelectrophoresis.

Latex agglutination take a look at for detection of Candida antigen in sufferers with disseminated illness.

A latex agglutination take a look at has been devised which permits detection of a circulating antigen in sufferers with systemic an infection attributable to Candida albicans, Candida tropicalis and Candida parapsilosis. Latex is sensitized with serum from rabbits immunized with complete warmth killed Candida albicans blastoconidia.
The lively part of this serum is IgG. Management latex, used to distinguish non-specific agglutination, is sensitized with the identical dilution of serum from a rabbit with out antibody to Candida species. Sera from plenty of affected person teams have been examined.
Whereas not one of the hundred regular controls had an antigen titer of larger than or equal to 1:4, 30 of 33 sufferers with documented disseminated candida an infection had antigen titers of 1:Four to 1:32. Two of the 33 gave false adverse outcomes, and one triggered nonspecific agglutination. In all sufferers who recovered after antifungal remedy antigen ranges returned to throughout the vary present in regular controls.

Comparability of staphylococcal coagglutinationlatex agglutination, and counterimmunoelectrophoresis for bacterial antigen detection.

Soluble antigens of Haemophilus influenzae kind b, Streptococcus pneumoniae, Neisseria meningitidis, and group B streptococcus have been regarded for in cerebrospinal fluid, serum, and urine through the use of the staphylococcal coagglutination take a look at, latex agglutination take a look at, and counterimmunoelectrophoresis.
The staphylococcal coaggultination and latex agglutination exams have been extra delicate than counterimmunoelectrophoresis in figuring out antigens of H. influenzae kind b, S. pneumoniae, and N. meningitidis. Not one of the three exams efficiently detected group B streptococcal antigens in physique fluids. Nonspecific reactions famous with the staphylococcal coagglutination take a look at may very well be normally eradicated after premixing take a look at specimens with soluble protein A.

Speedy slide latex agglutination take a look at for detection of methicillin resistance in Staphylococcus aureus.

The MRSA display take a look at (Denka Seiken Co., Ltd.), a commercially obtainable, fast (20-min) slide latex agglutination take a look at for the willpower of methicillin resistance by detection of PBP 2a in Staphylococcus aureus, was in contrast with the oxacillin agar display take a look at and PCR detection of the mecA gene. A complete of 563 S. aureus isolates have been examined. 200 ninety-six of the isolates have been methicillin-susceptible isolates from cultures of blood from consecutive sufferers.
Additionally, 267 methicillin-resistant isolates that comprised 248 completely different phage sorts have been examined. Methicillin resistance was outlined because the presence of the mecA gene. Of the 267 mecA gene-positive isolates, 263 have been optimistic by the MRSA display take a look at (sensitivity, 98.5%), and all of the mecA-gene adverse strains have been adverse by the MRSA display take a look at (specificity, 100%). The oxacillin agar display takes a look at detected methicillin resistance in 250 of the mecA gene-positive isolates (sensitivity, 93.6%).
The sensitivity of the MRSA display take a look at was statistically considerably greater than the sensitivity of the oxacillin agar display take a look at (P < 0. 05). The MRSA display take a look at is a extremely delicate and particular take a look at for the detection of methicillin resistance. Additionally, it provides outcomes inside half an hour and is straightforward to carry out, which makes this take a look at a helpful device within the ongoing battle towards methicillin-resistant S. aureus.

Analysis of systemic candidiasis by latex agglutination for serum antigen.

Three latex agglutination take a look at procedures for detecting Candida antigen in human serum have been in contrast in a retrospective research of 69 sufferers and 20 regular volunteers. Untreated human serum was reacted with two completely different latex reagents; one reagent additionally was reacted with serum handled with protease and warmth. The take a look at process with handled serum was finest, detecting serum antigen in 17 of 21 sufferers (81%) with disseminated candidiasis.
Judging by autopsy-proven instances, there was a rise in optimistic take a look at leads to the final 2 weeks of life. When untreated sera have been examined with this reagent, solely 3 (14%) of the 21 sufferers with disseminated candidiasis had detectable antigen in serum. A subset of those identical sera was examined by a industrial latex reagent (Candida Detection System lot C001; Ramco Laboratories, Inc., Houston, Tex.) and untreated serum.

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Description: Lectin from wheat germ (Triticum aestivum)

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Description: Lectin from wheat germ (Triticum aestivum)

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Wheat Germ Agglutinin, CF®680R Conjugate

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Description: Lectin from wheat germ (Triticum aestivum)

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Description: Lectin from wheat germ (Triticum aestivum)

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Wheat Germ Agglutinin, CF®405S Conjugate

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Description: Lectin from wheat germ (Triticum aestivum)

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Description: Lectin from wheat germ (Triticum aestivum)

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29028 5x1mg
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Description: Lectin from wheat germ (Triticum aestivum)

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Description: Lectin from wheat germ (Triticum aestivum)

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Of 18 sufferers with disseminated candidiasis, 5 (28%) had at the very least one optimistic serum. Sera from sufferers with much less extreme scientific types of candidiasis have been normally adverse whatever the take a look at process used. With one exception, sera from management sufferers have been adverse or have been optimistic solely in sera containing rheumatoid issue. Latex agglutination exams for Candida spp. in handled serum might show to be a helpful process for the fast analysis of extreme disseminated candidiasis.

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